Tag: biology cells practice test

Purification and assembly of thermostable Cy5 labeled γ-PNAs into a 3D DNA nanocage.

PNA is hybrid molecule ideally suited to bridging the useful panorama of polypeptides with the structural range that may be engineered with DNA nanostructures. Nonetheless, PNA could be more difficult to work with in aqueous solvents attributable to its hydrophobic nature. An answer part methodology utilizing pressure promoted, copper free click on chemistry was developed

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    • Cy3 and Cy5 dyes attached to oligonucleotide terminus stabilize DNA duplexes: predictive thermodynamic model.

    Cyanine dyes are vital chemical modifications of oligonucleotides exhibiting intensive and secure fluorescence at seen mild wavelengths. When Cy3 or Cy5 dye is connected to five’ finish of a DNA duplex, the dye stacks on the terminal base pair and stabilizes the duplex. Utilizing optical melting experiments, we’ve decided thermodynamic parameters that may predict the

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