化工 行业 现状 ChemDevelop

Purification and assembly of thermostable Cy5 labeled γ-PNAs into a 3D DNA nanocage.

PNA is hybrid molecule ideally suited to bridging the useful panorama of polypeptides with the structural range that may be engineered with DNA nanostructures. Nonetheless, PNA could be more difficult to work with in aqueous solvents attributable to its hydrophobic nature. An answer part methodology utilizing pressure promoted, copper free click on chemistry was developed to conjugate the fluorescent dye Cy5 to 2 bifunctional PNA strands as a primary step towards constructing cyclic PNA-polypeptides that may be organized inside 3D DNA nanoscaffolds. A 3D DNA nanocage was designed with binding websites for the two fluorescently labeled PNA strands in shut proximity to imitate protein energetic websites.

Denaturing polyacrylamide gel electrophoresis (PAGE) is launched as an environment friendly methodology for purifying charged, dye-labeled NA conjugates from giant excesses of unreacted dye and unreacted, impartial PNA. Elution from the gel in water was monitored by fluorescence and located to be extra environment friendly for the extra soluble PNA strand.

Native PAGE exhibits that each PNA strands hybridize to their supposed binding websites inside the DNA nanocage. Förster resonance power switch (FRET) with a Cy3 labeled DNA nanocage was used to find out the dissociation temperature of 1 PNA-Cy5 conjugate to be close to 50C. Regular-state and time resolved fluorescence was used to research the dye orientation and interactions inside the numerous complexes. Bifunctional, thermostable PNA molecules are intriguing candidates for controlling the meeting and orientation of peptides inside small DNA nanocages for mimicking protein catalytic websites.

FRET-Primarily based Mito-Particular Fluorescent Probe for Ratiometric Detection and Imaging of Endogenous Peroxynitrite: Dyad of Cy3 and Cy5.

Peroxynitrite (OONO(-)) is profoundly implicated in well being and illness. The physiological and pathological consequence of OONO(-) is said to its native focus, and therefore, a dependable OONO(-) assay is extremely desired. Now we have developed a FRET-based small-molecule fluorescent probe (PNCy3Cy5), harnessing the differential reactivity of Cy3 and Cy5 towards OONO(-) by fine-tuning.

It reveals excessive detection sensitivity and yields a ratiometric fluorescent sign. Now we have exemplified that it may be utilized in semiquantitative willpower of OONO(-) in residing cells. Notably, it particularly localizes in mitochondria, the place endogenous OONO(-) is predominantly generated. Thus, PNCy3Cy5 is a promising molecular instrument for peroxynitrite biology.

Cy5 complete protein normalization in Western blot evaluation.

Western blotting is a broadly used methodology for analyzing particular goal proteins in complicated protein samples. Housekeeping proteins are sometimes used for normalization to right for uneven pattern hundreds, however these require cautious validation since expression ranges might range with cell sort and therapy. We current a brand new, extra dependable methodology for normalization utilizing Cy5-prelabeled complete protein as a loading management. We used a prelabeling protocol based mostly on Cy5 N-hydroxysuccinimide ester labeling that produces a linear sign response.

We obtained a low coefficient of variation (CV) of seven% between the ratio of extracellular signal-regulated kinase (ERK1/2) goal to Cy5 complete protein management alerts over the entire loading vary from 2.5 to 20.0μg of Chinese language hamster ovary cell lysate protein. Corresponding experiments utilizing actin or tubulin as controls for normalization resulted in CVs of 13 and 18%, respectively.

Glyceraldehyde-3-phosphate dehydrogenase didn’t produce a proportional sign and was not appropriate for normalization in these cells. A comparability of ERK1/2 alerts from labeled and unlabeled samples confirmed that Cy5 prelabeling didn’t have an effect on antibody binding. Through the use of complete protein normalization we analyzed PP2A and Smad2/Three ranges with excessive confidence.

Microarray Scanner Efficiency Over a 5-Week Interval as Measured With Cy5 and Cy3 Serial Dilution Slides.

To research scanner efficiency and information improvement of an instrument qualification methodology, slides with replicates of successive dilutions of cyanine 5 (Cy5) and cyanine 3 (Cy3) dyes (known as dye slides) had been scanned. The successive dilutions type a dose-response curve from which efficiency could be assessed. The consequences of quite a lot of elements, together with the variety of scans and slide storage situations, on scanner efficiency over a five-week interval had been investigated and tracked with time sequence charts of dye sign depth, signal-to-noise (S/N), sign background, slope, and restrict of detection (LOD).

Scanner drift was tracked with a recognized steady reference materials, Customary Reference Materials (SRM) 2242. The best impact on the figures of benefit was the dye, with the Cy5 dye displaying indicators of degradation after one week of scanning impartial of all different elements whereas the Cy3 dye remained comparatively steady. Use of the charts to trace scanner efficiency over time holds promise for improvement of a technique for microarray scanner efficiency qualification. Though not a prescription for efficiency qualification, this introductory research gives adequate data concerning using dye slides to allow the person to institute a preliminary take a look at methodology.

Pre-labeling of various protein samples with a hard and fast quantity of Cy5 for sodium dodecyl sulfate-polyacrylamide gel electrophoresis evaluation.

A pre-labeling protocol based mostly on Cy5 N-hydroxysuccinimide (NHS) ester labeling of proteins has been developed for one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) evaluation. We present {that a} fastened quantity of sulfonated Cy5 can be utilized within the labeling response to label proteins over a broad focus range-more than three orders of magnitude. The optimum quantity of Cy5 was discovered to be 50 to 250pmol in 20μl utilizing a Tris-HCl labeling buffer at pH 8.7.

Labeling protein samples with a hard and fast quantity of dye on this vary balances the necessities of sub-nanogram detection sensitivity and low dye-to-protein (D/P) ratios for SDS-PAGE. Simulations of the labeling response reproduced experimental observations of each labeling kinetics and D/P ratios. Two-dimensional electrophoresis was used to look at the labeling of proteins in a cell lysate utilizing each sulfonated and non-sulfonated Cy5. For each sorts of Cy5, we noticed environment friendly labeling throughout a broad vary of molecular weights and isoelectric factors.

H-Aggregates of an Indocyanine Cy5 Dye: Transition from Robust to Weak Molecular Coupling.

The aggregation conduct of an Indocyanine Cy5 dye (2-[5-[1,1-dimethyl-3-(4-sulfobutyl)-1,3-dihydro-benzo[e]indol-2-ylidene]-penta-1,3-dienyl]-1,1-dimethyl-3-(4-sulfobutyl)-1H-benzo[e]indolium hydroxide, inside salt, sodium salt) in aqueous resolution is investigated utilizing absorption and fluorescence spectroscopies, in addition to cryogenic transmission electron microscopy (cryo-TEM).

The dye focus is assorted inside a broad vary from ∼1 μM to ∼10 mM. At average concentrations, typical H-aggregates are fashioned. After longer storage time, the absorption spectra of those options change dramatically. The attribute blue-shifted absorption band at round 600 nm turns into changed by a three-banded absorption spectrum, which spreads over a large wavelength vary of ∼600 as much as 800 nm.

DBF4 (Protein DBF4 Homolog A, Activator of S Phase Kinase, DBF4-type Zinc Finger-containing Protein 1, Chiffon Homolog A, ASK, DBF4A, ZDBF1) (FITC)
MBS6375680-01mL	MyBiosource	0.1mL	EUR 920

DBF4 (Protein DBF4 Homolog A, Activator of S Phase Kinase, DBF4-type Zinc Finger-containing Protein 1, Chiffon Homolog A, ASK, DBF4A, ZDBF1) (FITC)
MBS6375680-5x01mL	MyBiosource	5x0.1mL	EUR 3990

DBF4 (Protein DBF4 Homolog A, Activator of S Phase Kinase, DBF4-type Zinc Finger-containing Protein 1, Chiffon Homolog A, ASK, DBF4A, ZDBF1) (HRP)
MBS6375681-01mL	MyBiosource	0.1mL	EUR 920

DBF4 (Protein DBF4 Homolog A, Activator of S Phase Kinase, DBF4-type Zinc Finger-containing Protein 1, Chiffon Homolog A, ASK, DBF4A, ZDBF1) (HRP)
MBS6375681-5x01mL	MyBiosource	5x0.1mL	EUR 3990

DBF4 (Protein DBF4 Homolog A, Activator of S Phase Kinase, DBF4-type Zinc Finger-containing Protein 1, Chiffon Homolog A, ASK, DBF4A, ZDBF1) (PE)
MBS6375687-01mL	MyBiosource	0.1mL	EUR 920

DBF4 (Protein DBF4 Homolog A, Activator of S Phase Kinase, DBF4-type Zinc Finger-containing Protein 1, Chiffon Homolog A, ASK, DBF4A, ZDBF1) (PE)
MBS6375687-5x01mL	MyBiosource	5x0.1mL	EUR 3990

DBF4 (Protein DBF4 Homolog A, Activator of S Phase Kinase, DBF4-type Zinc Finger-containing Protein 1, Chiffon Homolog A, ASK, DBF4A, ZDBF1) (FITC)
MBS6146776-01mL	MyBiosource	0.1(mL	EUR 875

DBF4 (Protein DBF4 Homolog A, Activator of S Phase Kinase, DBF4-type Zinc Finger-containing Protein 1, Chiffon Homolog A, ASK, DBF4A, ZDBF1) (FITC)
MBS6146776-5x01mL	MyBiosource	5x0.1mL	EUR 3800

DBF4 (Protein DBF4 Homolog A, Activator of S Phase Kinase, DBF4-type Zinc Finger-containing Protein 1, Chiffon Homolog A, ASK, DBF4A, ZDBF1) (PE)
MBS6157382-01mL	MyBiosource	0.1(mL	EUR 875

DBF4 (Protein DBF4 Homolog A, Activator of S Phase Kinase, DBF4-type Zinc Finger-containing Protein 1, Chiffon Homolog A, ASK, DBF4A, ZDBF1) (PE)
MBS6157382-5x01mL	MyBiosource	5x0.1mL	EUR 3800

DBF4 (Protein DBF4 Homolog A, Activator of S Phase Kinase, DBF4-type Zinc Finger-containing Protein 1, Chiffon Homolog A, ASK, DBF4A, ZDBF1) (HRP)
MBS6152079-01mL	MyBiosource	0.1(mL	EUR 875

DBF4 (Protein DBF4 Homolog A, Activator of S Phase Kinase, DBF4-type Zinc Finger-containing Protein 1, Chiffon Homolog A, ASK, DBF4A, ZDBF1) (HRP)
MBS6152079-5x01mL	MyBiosource	5x0.1mL	EUR 3800

DBF4 (Protein DBF4 Homolog A, Activator of S Phase Kinase, DBF4-type Zinc Finger-containing Protein 1, Chiffon Homolog A, ASK, DBF4A, ZDBF1) (Biotin)
MBS6141473-01mL	MyBiosource	0.1(mL	EUR 875

DBF4 (Protein DBF4 Homolog A, Activator of S Phase Kinase, DBF4-type Zinc Finger-containing Protein 1, Chiffon Homolog A, ASK, DBF4A, ZDBF1) (Biotin)
MBS6141473-5x01mL	MyBiosource	5x0.1mL	EUR 3800

DBF4 (Protein DBF4 Homolog A, Activator of S Phase Kinase, DBF4-type Zinc Finger-containing Protein 1, Chiffon Homolog A, ASK, DBF4A, ZDBF1) (Biotin)
MBS6375679-01mL	MyBiosource	0.1mL	EUR 920

DBF4 (Protein DBF4 Homolog A, Activator of S Phase Kinase, DBF4-type Zinc Finger-containing Protein 1, Chiffon Homolog A, ASK, DBF4A, ZDBF1) (Biotin)
MBS6375679-5x01mL	MyBiosource	5x0.1mL	EUR 3990

DBF4 (Protein DBF4 Homolog A, Activator of S Phase Kinase, DBF4-type Zinc Finger-containing Protein 1, Chiffon Homolog A, ASK, DBF4A, ZDBF1) (MaxLight 405)
MBS6375682-01mL	MyBiosource	0.1mL	EUR 920

DBF4 (Protein DBF4 Homolog A, Activator of S Phase Kinase, DBF4-type Zinc Finger-containing Protein 1, Chiffon Homolog A, ASK, DBF4A, ZDBF1) (MaxLight 405)
MBS6375682-5x01mL	MyBiosource	5x0.1mL	EUR 3990

DBF4 (Protein DBF4 Homolog A, Activator of S Phase Kinase, DBF4-type Zinc Finger-containing Protein 1, Chiffon Homolog A, ASK, DBF4A, ZDBF1) (MaxLight 490)
MBS6375683-01mL	MyBiosource	0.1mL	EUR 920

DBF4 (Protein DBF4 Homolog A, Activator of S Phase Kinase, DBF4-type Zinc Finger-containing Protein 1, Chiffon Homolog A, ASK, DBF4A, ZDBF1) (MaxLight 490)
MBS6375683-5x01mL	MyBiosource	5x0.1mL	EUR 3990

DBF4 (Protein DBF4 Homolog A, Activator of S Phase Kinase, DBF4-type Zinc Finger-containing Protein 1, Chiffon Homolog A, ASK, DBF4A, ZDBF1) (MaxLight 550)
MBS6375684-01mL	MyBiosource	0.1mL	EUR 920

DBF4 (Protein DBF4 Homolog A, Activator of S Phase Kinase, DBF4-type Zinc Finger-containing Protein 1, Chiffon Homolog A, ASK, DBF4A, ZDBF1) (MaxLight 550)
MBS6375684-5x01mL	MyBiosource	5x0.1mL	EUR 3990

DBF4 (Protein DBF4 Homolog A, Activator of S Phase Kinase, DBF4-type Zinc Finger-containing Protein 1, Chiffon Homolog A, ASK, DBF4A, ZDBF1) (MaxLight 650)
MBS6375685-01mL	MyBiosource	0.1mL	EUR 920

DBF4 (Protein DBF4 Homolog A, Activator of S Phase Kinase, DBF4-type Zinc Finger-containing Protein 1, Chiffon Homolog A, ASK, DBF4A, ZDBF1) (MaxLight 650)
MBS6375685-5x01mL	MyBiosource	5x0.1mL	EUR 3990

DBF4 (Protein DBF4 Homolog A, Activator of S Phase Kinase, DBF4-type Zinc Finger-containing Protein 1, Chiffon Homolog A, ASK, DBF4A, ZDBF1) (MaxLight 750)
MBS6375686-01mL	MyBiosource	0.1mL	EUR 920

DBF4 (Protein DBF4 Homolog A, Activator of S Phase Kinase, DBF4-type Zinc Finger-containing Protein 1, Chiffon Homolog A, ASK, DBF4A, ZDBF1) (MaxLight 750)
MBS6375686-5x01mL	MyBiosource	5x0.1mL	EUR 3990

DBF4 (Protein DBF4 Homolog A, Activator of S Phase Kinase, DBF4-type Zinc Finger-containing Protein 1, Chiffon Homolog A, ASK, DBF4A, ZDBF1) (MaxLight 490)
MBS6200405-01mL	MyBiosource	0.1(mL	EUR 875

DBF4 (Protein DBF4 Homolog A, Activator of S Phase Kinase, DBF4-type Zinc Finger-containing Protein 1, Chiffon Homolog A, ASK, DBF4A, ZDBF1) (MaxLight 490)
MBS6200405-5x01mL	MyBiosource	5x0.1mL	EUR 3800

DBF4 (Protein DBF4 Homolog A, Activator of S Phase Kinase, DBF4-type Zinc Finger-containing Protein 1, Chiffon Homolog A, ASK, DBF4A, ZDBF1) (MaxLight 550)
MBS6211080-01mL	MyBiosource	0.1(mL	EUR 875

DBF4 (Protein DBF4 Homolog A, Activator of S Phase Kinase, DBF4-type Zinc Finger-containing Protein 1, Chiffon Homolog A, ASK, DBF4A, ZDBF1) (MaxLight 550)
MBS6211080-5x01mL	MyBiosource	5x0.1mL	EUR 3800

DBF4 (Protein DBF4 Homolog A, Activator of S Phase Kinase, DBF4-type Zinc Finger-containing Protein 1, Chiffon Homolog A, ASK, DBF4A, ZDBF1) (MaxLight 405)
MBS6189730-01mL	MyBiosource	0.1(mL	EUR 875

DBF4 (Protein DBF4 Homolog A, Activator of S Phase Kinase, DBF4-type Zinc Finger-containing Protein 1, Chiffon Homolog A, ASK, DBF4A, ZDBF1) (MaxLight 405)
MBS6189730-5x01mL	MyBiosource	5x0.1mL	EUR 3800

DBF4 (Protein DBF4 Homolog A, Activator of S Phase Kinase, DBF4-type Zinc Finger-containing Protein 1, Chiffon Homolog A, ASK, DBF4A, ZDBF1) (MaxLight 650)
MBS6221755-01mL	MyBiosource	0.1(mL	EUR 875

DBF4 (Protein DBF4 Homolog A, Activator of S Phase Kinase, DBF4-type Zinc Finger-containing Protein 1, Chiffon Homolog A, ASK, DBF4A, ZDBF1) (MaxLight 650)
MBS6221755-5x01mL	MyBiosource	5x0.1mL	EUR 3800

DBF4 (Protein DBF4 Homolog A, Activator of S Phase Kinase, DBF4-type Zinc Finger-containing Protein 1, Chiffon Homolog A, ASK, DBF4A, ZDBF1) (MaxLight 750)
MBS6232430-01mL	MyBiosource	0.1(mL	EUR 875

DBF4 (Protein DBF4 Homolog A, Activator of S Phase Kinase, DBF4-type Zinc Finger-containing Protein 1, Chiffon Homolog A, ASK, DBF4A, ZDBF1) (MaxLight 750)
MBS6232430-5x01mL	MyBiosource	5x0.1mL	EUR 3800

DBF4 Antibody
8C11821	AAT Bioquest	50ug	EUR 368
Description: DBF4 Antibody

DBF4 antibody
10R-1284	Fitzgerald	100 ug	EUR 614.4
Description: Mouse monoclonal DBF4 antibody

DBF4 Antibody
34095	SAB	100ul	EUR 319

DBF4 Antibody
34095-100ul	SAB	100ul	EUR 302.4

DBF4 Antibody
34095-50ul	SAB	50ul	EUR 224.4

DBF4 Antibody
1-CSB-PA887010LA01HU	Cusabio	Ask for price Ask for price	100ug 50ug
Description: A polyclonal antibody against DBF4. Recognizes DBF4 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200

DBF4 Antibody
E034095	EnoGene	100μg/100μl	EUR 255
Description: Available in various conjugation types.

DBF4 Antibody
E11-11821C	EnoGene	100μg	EUR 225
Description: Available in various conjugation types.

DBF4 Antibody
E19-3462	EnoGene	100μg/100μl	EUR 225
Description: Available in various conjugation types.

DBF4 Antibody
DF3462	Affbiotech	200ul	EUR 420

DBF4 Antibody
DF3462-100ul	Affinity Biosciences	100ul	EUR 280

DBF4 Antibody
DF3462-200ul	Affinity Biosciences	200ul	EUR 350

DBF4 Antibody
E38PA2519	EnoGene	100ul	EUR 225
Description: Available in various conjugation types.

DBF4 Antibody
CAC11222-100ug	Biomatik Corporation	100ug	EUR 314

DBF4 Antibody
CAC11222-50ug	Biomatik Corporation	50ug	EUR 199.2

DBF4 antibody
70R-16745	Fitzgerald	50 ul	EUR 289
Description: Rabbit polyclonal DBF4 antibody

DBF4 antibody
70R-32181	Fitzgerald	100 ug	EUR 294
Description: Rabbit polyclonal DBF4 antibody

DBF4 antibody
70R-50838	Fitzgerald	100 ul	EUR 242
Description: Purified Polyclonal DBF4 antibody

DBF4 Antibody
1-CSB-PA006516GA01HU	Cusabio	Ask for price Ask for price	150ul 50ul
Description: A polyclonal antibody against DBF4. Recognizes DBF4 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB

DBF4 Antibody
CSB-PA113425-	Cusabio	each	EUR 402
Description: A polyclonal antibody against DBF4. Recognizes DBF4 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

DBF4 Antibody
CSB-PA113425-100ul	Cusabio	100ul	EUR 379.2
Description: A polyclonal antibody against DBF4. Recognizes DBF4 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

DBF4 Antibody
1-CSB-PA002079	Cusabio	Ask for price Ask for price	100ug 50ug
Description: A polyclonal antibody against DBF4. Recognizes DBF4 from Human, Mouse, Monkey. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/10000

DBF4 Antibody
ABD3462	Nova Lifetech	100ug	EUR 325

Dbf4 Antibody
GWB-MS534C	GenWay Biotech	50ug	Ask for price

Dbf4 Antibody
R33506-100UG	NSJ Bioreagents	100 ug	EUR 339.15
Description: Additional name(s) for this target protein: Dumbbell former 4

DBF4 Antibody
RQ5689	NSJ Bioreagents	100 ug	EUR 356.15
Description: Using both gain- and loss-of-function experiments, it was found that Xenopus Dbf4 inhibited canonical Wnt signaling. Depletion of endogenous Dbf4 did not disturb gastrulation movements or early organizer genes, but resulted in embryos with morphologically defective heart and eyes and suppressed cardiac markers. The function of Dbf4 in heart development appeared to be independent of its role in the cell cycle. Dbf4 interacted physically and functionally with Frodo, a context-dependent regulator of Wnt signaling.

DBF4 Antibody
MBS7105433-005mg	MyBiosource	0.05mg	EUR 190

DBF4 Antibody
MBS7105433-01mg	MyBiosource	0.1mg	EUR 270

DBF4 Antibody
MBS7105433-5x01mg	MyBiosource	5x0.1mg	EUR 1205

DBF4 Antibody
MBS7130989-01mL	MyBiosource	0.1mL	EUR 270

DBF4 Antibody
MBS7130989-5x01mL	MyBiosource	5x0.1mL	EUR 1200

DBF4 Antibody
MBS7116219-005mg	MyBiosource	0.05mg	EUR 150

DBF4 Antibody
MBS7116219-01mg	MyBiosource	0.1mg	EUR 190

DBF4 Antibody
MBS7116219-5x01mg	MyBiosource	5x0.1mg	EUR 810

DBF4 Antibody
MBS9411648-005mL	MyBiosource	0.05mL	EUR 245

DBF4 Antibody
MBS9411648-01mL	MyBiosource	0.1mL	EUR 305

DBF4 Antibody
MBS9411648-5x01mL	MyBiosource	5x0.1mL	EUR 1230

DBF4 Antibody
MBS9204412-01mL	MyBiosource	0.1mL	EUR 415

DBF4 Antibody
MBS9204412-5x01mL	MyBiosource	5x0.1mL	EUR 1841

DBF4 Antibody
MBS8581369-01mL	MyBiosource	0.1mL	EUR 305

DBF4 Antibody
MBS8581369-01mLAF405L	MyBiosource	0.1mL(AF405L)	EUR 465

DBF4 Antibody
MBS8581369-01mLAF405S	MyBiosource	0.1mL(AF405S)	EUR 465

DBF4 Antibody
MBS8581369-01mLAF610	MyBiosource	0.1mL(AF610)	EUR 465

DBF4 Antibody
MBS8581369-01mLAF635	MyBiosource	0.1mL(AF635)	EUR 465

DBF4 Antibody
MBS855166-01mg	MyBiosource	0.1mg	EUR 305

DBF4 Antibody
MBS855166-01mLAF405L	MyBiosource	0.1mL(AF405L)	EUR 465

DBF4 Antibody
MBS855166-01mLAF405S	MyBiosource	0.1mL(AF405S)	EUR 465

DBF4 Antibody
MBS855166-01mLAF610	MyBiosource	0.1mL(AF610)	EUR 465

DBF4 Antibody
MBS855166-01mLAF635	MyBiosource	0.1mL(AF635)	EUR 465

DBF4 Antibody
MBS8514195-01mg	MyBiosource	0.1mg	EUR 305

DBF4 Antibody
MBS8514195-01mLAF405L	MyBiosource	0.1mL(AF405L)	EUR 465

DBF4 Antibody
MBS8514195-01mLAF405S	MyBiosource	0.1mL(AF405S)	EUR 465

DBF4 Antibody
MBS8514195-01mLAF610	MyBiosource	0.1mL(AF610)	EUR 465

DBF4 Antibody
MBS8514195-01mLAF635	MyBiosource	0.1mL(AF635)	EUR 465

DBF4 Antibody
MBS5315456-01mL	MyBiosource	0.1mL	EUR 470

DBF4 Antibody
MBS5315456-5x01mL	MyBiosource	5x0.1mL	EUR 1955

DBF4 Antibody
MBS9604921-01mL	MyBiosource	0.1mL	EUR 260

DBF4 Antibody
MBS9604921-02mL	MyBiosource	0.2mL	EUR 305

DBF4 Antibody
MBS9604921-5x02mL	MyBiosource	5x0.2mL	EUR 1220

DBF4 Antibody
MBS9505514-005mL	MyBiosource	0.05mL	EUR 285

DBF4 Antibody
MBS9505514-01mL	MyBiosource	0.1mL	EUR 385

DBF4 Antibody
MBS9505514-5x01mL	MyBiosource	5x0.1mL	EUR 1590

Dbf4 Antibody
C11821-100ul	Assay Biotech	100μl	EUR 217
Description: Dbf4 Rabbit Polyclonal Antibody

Dbf4 Antibody
C11821-50ul	Assay Biotech	50μl	EUR 143.5
Description: Dbf4 Rabbit Polyclonal Antibody

DBF4B Antibody
1-CSB-PA822784LA01HU	Cusabio	Ask for price Ask for price	100ug 50ug
Description: A polyclonal antibody against DBF4B. Recognizes DBF4B from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC; Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200

DBF4B Antibody
CAC10496-100ug	Biomatik Corporation	100ug	EUR 314

DBF4B Antibody
CAC10496-50ug	Biomatik Corporation	50ug	EUR 199.2

DBF4B Antibody
MBS1495733-005mg	MyBiosource	0.05mg	EUR 190

DBF4B Antibody
MBS1495733-01mg	MyBiosource	0.1mg	EUR 270

DBF4B Antibody
MBS1495733-5x01mg	MyBiosource	5x0.1mg	EUR 1205

Protein DBF4 Homolog A (DBF4) Antibody
20-abx111962	Abbexa	Ask for price Ask for price	150 ul 50 ul

Protein DBF4 Homolog A (DBF4) Antibody
20-abx008298	Abbexa	Ask for price Ask for price Ask for price	100 ul 200 ul 30 ul

Protein DBF4 Homolog A (DBF4) Antibody
20-abx013828	Abbexa	Ask for price Ask for price Ask for price Ask for price	100 ug 10 ug 200 ug 300 µg

Protein DBF4 Homolog A (DBF4) Antibody
abx232255-100ug	Abbexa	100 ug	EUR 610.8

Protein DBF4 Homolog A (DBF4) Antibody
20-abx338497	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 1 mg 200 ug 20 ug 50 ug

Protein DBF4 Homolog A (DBF4) Antibody
20-abx326302	Abbexa	Ask for price Ask for price	100 ug 50 ug

Protein DBF4 Homolog A (DBF4) Antibody
abx331894-100ul	Abbexa	100 ul	EUR 510

Protein DBF4 Homolog A (Dbf4) Antibody
abx431868-200ul	Abbexa	200 ul	EUR 460.8

Protein DBF4 Homolog A (DBF4) Antibody
abx111962-100l	Abbexa	100 µl	EUR 612.5

Protein DBF4 Homolog A (DBF4) Antibody
abx232255-100g	Abbexa	100 µg	EUR 350

Protein DBF4 Homolog A (DBF4) Antibody
abx338497-100g	Abbexa	100 µg	EUR 162.5

Protein DBF4 Homolog A (DBF4) Antibody
abx402024-100g	Abbexa	100 µg	EUR 287.5

Protein DBF4 Homolog A (Dbf4) Antibody
abx431868-200l	Abbexa	200 µl	EUR 387.5

Protein DBF4 Homolog A (DBF4) Antibody
abx326302-100g	Abbexa	100 µg	EUR 250

Protein DBF4 Homolog A (DBF4) Antibody
abx326302-50g	Abbexa	50 µg	EUR 187.5

Protein DBF4 Homolog A (DBF4) Antibody
abx013828-100l	Abbexa	100 µl	EUR 43.75

Protein DBF4 Homolog B (DBF4B) Antibody
20-abx302801	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 1 mg 200 ug 20 ug 50 ug

Protein DBF4 Homolog B (DBF4B) Antibody
abx432593-200ul	Abbexa	200 ul	EUR 343.2

Protein DBF4 Homolog B (DBF4B) Antibody
abx302801-100g	Abbexa	100 µg	EUR 362.5

Protein DBF4 Homolog B (DBF4B) Antibody
abx302801-20g	Abbexa	20 µg	EUR 162.5

Protein DBF4 Homolog B (DBF4B) Antibody
abx302801-50g	Abbexa	50 µg	EUR 250

Protein DBF4 Homolog B (DBF4B) Antibody
abx432593-100g	Abbexa	100 µg	EUR 250

anti- DBF4 antibody
FNab02255	FN Test	100µg	EUR 658.5
Description: Antibody raised against DBF4

Protein DBF4 Homolog A (DBF4) Antibody (HRP)
20-abx337243	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 1 mg 200 ug 20 ug 50 ug

Protein DBF4 Homolog A (DBF4) Antibody (HRP)
abx337243-100g	Abbexa	100 µg	EUR 362.5

Protein DBF4 Homolog A (DBF4) Antibody (HRP)
abx337243-20g	Abbexa	20 µg	EUR 162.5

Protein DBF4 Homolog A (DBF4) Antibody (HRP)
abx337243-50g	Abbexa	50 µg	EUR 250

Protein DBF4 Homolog A (DBF4) Antibody (FITC)
20-abx337244	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 1 mg 200 ug 20 ug 50 ug

Protein DBF4 Homolog A (DBF4) Antibody (FITC)
abx337244-100g	Abbexa	100 µg	EUR 362.5

Protein DBF4 Homolog A (DBF4) Antibody (FITC)
abx337244-20g	Abbexa	20 µg	EUR 162.5

Protein DBF4 Homolog A (DBF4) Antibody (FITC)
abx337244-50g	Abbexa	50 µg	EUR 250

DBF4B Antibody (HRP)
20-abx309042	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 1 mg 200 ug 20 ug 50 ug

DBF4B Antibody (HRP)
abx309042-100g	Abbexa	100 µg	EUR 362.5

DBF4B Antibody (HRP)
abx309042-20g	Abbexa	20 µg	EUR 162.5

DBF4B Antibody (HRP)
abx309042-50g	Abbexa	50 µg	EUR 250

DBF4B Antibody (FITC)
20-abx309043	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 1 mg 200 ug 20 ug 50 ug

DBF4B Antibody (FITC)
abx309043-100g	Abbexa	100 µg	EUR 362.5

DBF4B Antibody (FITC)
abx309043-20g	Abbexa	20 µg	EUR 162.5

DBF4B Antibody (FITC)
abx309043-50g	Abbexa	50 µg	EUR 250

Protein DBF4 Homolog A (DBF4) Antibody (Biotin)
20-abx337245	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 1 mg 200 ug 20 ug 50 ug

Protein DBF4 Homolog A (DBF4) Antibody (Biotin)
abx337245-100g	Abbexa	100 µg	EUR 362.5

Protein DBF4 Homolog A (DBF4) Antibody (Biotin)
abx337245-20g	Abbexa	20 µg	EUR 162.5

Protein DBF4 Homolog A (DBF4) Antibody (Biotin)
abx337245-50g	Abbexa	50 µg	EUR 250

DBF4B Antibody (Biotin)
20-abx309044	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 1 mg 200 ug 20 ug 50 ug

DBF4B Antibody (Biotin)
abx309044-100g	Abbexa	100 µg	EUR 362.5

DBF4B Antibody (Biotin)
abx309044-20g	Abbexa	20 µg	EUR 162.5

DBF4B Antibody (Biotin)
abx309044-50g	Abbexa	50 µg	EUR 250

Dbf4 Polyclonal Antibody
ABP51149-003ml	Abbkine	0.03ml	EUR 189.6
Description: A polyclonal antibody for detection of Dbf4 from Human, Mouse, Monkey. This Dbf4 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human Dbf4 at AA range: 10-90

Dbf4 Polyclonal Antibody
ABP51149-01ml	Abbkine	0.1ml	EUR 346.8
Description: A polyclonal antibody for detection of Dbf4 from Human, Mouse, Monkey. This Dbf4 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human Dbf4 at AA range: 10-90

Dbf4 Polyclonal Antibody
ABP51149-02ml	Abbkine	0.2ml	EUR 496.8
Description: A polyclonal antibody for detection of Dbf4 from Human, Mouse, Monkey. This Dbf4 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human Dbf4 at AA range: 10-90

Dbf4 Polyclonal Antibody
E20-71295	EnoGene	100ug	EUR 225
Description: Available in various conjugation types.

Polyclonal DBF4 Antibody
APR05521G	Leading Biology	0.1ml	EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human DBF4 . This antibody is tested and proven to work in the following applications:

DBF4 Conjugated Antibody
C34095	SAB	100ul	EUR 476.4

Dbf4 Polyclonal Antibody
E44H04492	EnoGene	100ul	EUR 255
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

Dbf4 Polyclonal Antibody
BT-AP02506-100ul	Jiaxing Korain Biotech Ltd (BT Labs)	100ul	Ask for price
Description: The Dbf4/Cdc7 protein kinase is essential for the activation of replication origins during S phase. Cdc7-Dbf4 efficiently phosphorylates several proteins that are required for the initiation of DNA replication, including five of the six minichromosome maintenance (Mcm) proteins and the p180 subunit of DNA polymerase α-primase. This protein complex consists of the catalytic subunit Cdc7 associating with the regulatory and activating subunit Dbf4, and the kinase activity of the complex is regulated throughout the cell cycle mainly by fluctuating levels of Dbf4. Cdc7 is consistently expressed throughout the cell cycle, while the expression of Dbf4 is absent during G1 phase and accumulates during S and G2 phases. The anaphase-promoting complex rapidly degrades Dbf4 at the time of chromosome segregation, and the stability of Dbf4 remains low during pre-Start G1 phase. The coordinated degradation of Dbf4 and the time of chromosomes separation is important to ensuring that prereplicative complexes, which assemble after chromosome segregation, do not immediately refire.

Dbf4 Polyclonal Antibody
BT-AP02506-20ul	Jiaxing Korain Biotech Ltd (BT Labs)	20ul	Ask for price
Description: The Dbf4/Cdc7 protein kinase is essential for the activation of replication origins during S phase. Cdc7-Dbf4 efficiently phosphorylates several proteins that are required for the initiation of DNA replication, including five of the six minichromosome maintenance (Mcm) proteins and the p180 subunit of DNA polymerase α-primase. This protein complex consists of the catalytic subunit Cdc7 associating with the regulatory and activating subunit Dbf4, and the kinase activity of the complex is regulated throughout the cell cycle mainly by fluctuating levels of Dbf4. Cdc7 is consistently expressed throughout the cell cycle, while the expression of Dbf4 is absent during G1 phase and accumulates during S and G2 phases. The anaphase-promoting complex rapidly degrades Dbf4 at the time of chromosome segregation, and the stability of Dbf4 remains low during pre-Start G1 phase. The coordinated degradation of Dbf4 and the time of chromosomes separation is important to ensuring that prereplicative complexes, which assemble after chromosome segregation, do not immediately refire.

Dbf4 Polyclonal Antibody
BT-AP02506-50ul	Jiaxing Korain Biotech Ltd (BT Labs)	50ul	Ask for price
Description: The Dbf4/Cdc7 protein kinase is essential for the activation of replication origins during S phase. Cdc7-Dbf4 efficiently phosphorylates several proteins that are required for the initiation of DNA replication, including five of the six minichromosome maintenance (Mcm) proteins and the p180 subunit of DNA polymerase α-primase. This protein complex consists of the catalytic subunit Cdc7 associating with the regulatory and activating subunit Dbf4, and the kinase activity of the complex is regulated throughout the cell cycle mainly by fluctuating levels of Dbf4. Cdc7 is consistently expressed throughout the cell cycle, while the expression of Dbf4 is absent during G1 phase and accumulates during S and G2 phases. The anaphase-promoting complex rapidly degrades Dbf4 at the time of chromosome segregation, and the stability of Dbf4 remains low during pre-Start G1 phase. The coordinated degradation of Dbf4 and the time of chromosomes separation is important to ensuring that prereplicative complexes, which assemble after chromosome segregation, do not immediately refire.

Dbf4 Polyclonal Antibody
MBS9243372-01mL	MyBiosource	0.1mL	EUR 415

Dbf4 Polyclonal Antibody
MBS9243372-5x01mL	MyBiosource	5x0.1mL	EUR 1841

Dbf4 Polyclonal Antibody
MBS8522276-01mg	MyBiosource	0.1mg	EUR 305

Nonetheless, on the highest dye focus and within the presence of ∼(10 to 30) mM NaCl, the three-banded spectrum is noticed instantly after preparation. The spectroscopic options could be ascribed to a structural transformation of strongly to weakly coupled H-type aggregates. The transformation is promoted by a rise of the ionic energy. Cryo-TEM information reveal that the weakly coupled H’-aggregates are organized in well-ordered, prolonged monolayer sheets, whereas the strongly coupled H-aggregates seem to include particles of only some nanometers in measurement.