Optical assay for glucose based on the luminescnence decay time of the long wavelength dye Cy5™.
An optical assay for glucose is described primarily based on the luminescence decay time of a protracted wavelength dye (Cy5) which might be excited with at the moment out there crimson laser diodes. Concanavalin A was covalently labeled with Cy5 which served because the donor in an assay primarily based on fluorescence resonance power switch (FRET).
The acceptor was Malachite Inexperienced which was covalently linked to insulin which served as a provider protein. To supply binding affinity for ConA Malachite Inexperienced insulin was additionally covalently labeled with maltose (MIMG). Binding of Cy5ConA to MIMG resulted in a decreased depth and decay time of Cy5 as noticed by time-correlated single photon counting. Glucose was detected by aggressive displacement of MIMG from Cy5ConA, leading to elevated depth and decay time.
This glucose assay has a number of options which can lead to sensible actual world assays for glucose. The lengthy absorption wavelength of Cy5 permits excitation with crimson laser diodes, which might be readily pulsed or amplitude-modulated for time-domain or frequency-domain decay time measurements. Moreover, decay occasions might be measured via pores and skin utilizing lengthy wavelength excitation and emission, suggesting the potential for an implanted glucose sensor. And at last, the assay affinity and reversibility can in precept be adjusted by controlling the extent and kind of sugar labeling of the provider protein.
Labeling Antibodies with Cy5-Phycoerythrin.
Conjugates of the FRET dye Cy5-phycoerythrin (Cy5PE) with antibodies are comparatively easy to make. The protocol does require synthesis of the Cy5PE tandem dye. Phycoerythrin (PE) might be bought from a number of distributors. This sort of conjugate is helpful for immunofluorescence research involving protein targets with low expression ranges.
Though your entire conjugation might be carried out in a single day, there’s an in a single day stopping level. When initially making Cy5PE derivatives, a number of completely different conjugates with various ratios of Cy5 to PE must be made. These must be examined by conjugating to a well-characterized antibody. Absorbance spectra readings are a really worthwhile step to find out the standard of the Cy5PE label.
Carboxylated Cy5-Labeled Comb Polymers Passively Diffuse the Cell Membrane and Goal Mitochondria.
An in depth understanding of the mobile uptake and trafficking of nanomaterials is important for the design of “sensible” intracellular drug supply autos. Sometimes, mobile interactions might be tailor-made by endowing supplies with particular properties, for instance, via the introduction of expenses or concentrating on teams. On this examine, water-soluble carboxylated N-acylated poly(amino ester)-based comb polymers of various diploma of polymerization and side-chain modification had been synthesized through a mix of spontaneous zwitterionic copolymerization and redox-initiated reversible addition-fragmentation chain-transfer polymerization and absolutely characterised by 1H NMR spectroscopy and measurement exclusion chromatography. The comb polymers confirmed no cell toxicity towards NIH/3T3 and N27 cell strains nor hemolysis.
Detailed mobile affiliation and uptake research by circulation cytometry and confocal laser scanning microscopy (CLSM) revealed that the carboxylated polymers had been able to passively diffusing cell membranes and concentrating on mitochondria. The interaction of pendant carboxylic acids of the comb polymers and the Cy5-label was recognized as main driving power for this conduct, which was demonstrated to be relevant in NIH/3T3 and N27 cell strains.
Blocking of the carboxylic acids via modification with 2-methoxyethylamine and poly(2-ethyl-2-oxazoline) or substitute of the dye label with a special dye (e.g., fluorescein) resulted in an alteration of the mobile uptake mechanism towards endocytosis as demonstrated by CLSM. In distinction, partial modification of the carboxylic acid teams allowed to retain the mobile interplay, therefore, rendering these comb polymers a extremely practical mitochondria focused provider platform for future drug supply functions and imaging functions.
Ab Initio Research of Exciton Interactions of Cy5 Dyes.
The excited state properties of cyanine dyes and the orientations of their aggregates had been studied utilizing density practical idea (DFT). The consequences of exchange-correlation practical and solvent mannequin on the absorption spectrum of Cy5 was investigated. Utilizing the 6-31+G(d,p) foundation set and B3LYP exchange-correlation practical with IEF-PCM (water) solvent, the expected spectrum achieved a most absorbance inside 0.007 eV of experiment.
An in-house program primarily based on the theoretical mannequin of Kühn, Renger, and Could (KRM), which predicts the orientation of dyes inside an combination from its absorbance and round dichroism (CD) spectra or vice versa, was used to analyze the orientation of an experimentally noticed dimer. The absorbance spectrum predicted utilizing the KRM mannequin of the dimer construction optimized with the 6-31+G(d,p) foundation set, ωB97XD exchange-correlation practical, and IEF-PCM solvent agrees with experimental knowledge.
dUTPs conjugated with zwitterionic Cy3 or Cy5 fluorophore analogues are efficient substrates for DNA amplification and labelling by Taq polymerase.
To develop structural modifications of dNTPs which might be suitable with Taq DNA polymerase exercise, we synthesized eight dUTP derivatives conjugated with Cy3 or Cy5 dye analogues that differed in cost and cost distribution all through the fluorophore.
These dUTP derivatives and industrial Cy3- and Cy5-dUTP had been studied in Taq polymerase-dependent polymerase chain reactions (PCRs) and in primer extension reactions utilizing mannequin templates containing one, two and three adjoining adenine nucleotides.
The relative quantities of amplified DNA and the kinetic parameters Km and Vmax characterizing the incorporation of labelled dUMPs have been estimated utilizing fluorescence measurements and analysed. The dUTPs labelled with electroneutral zwitterionic analogues of Cy3 or Cy5 fluorophores had been utilized by Taq polymerase roughly one order of magnitude extra successfully than the dUTPs labelled with negatively charged analogues of Cy3 or Cy5.
Checkpoint Kinase 1 Phospho-Ser296 (CHEK1 pS296) Antibody |
20-abx324716 |
Abbexa |
-
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|
|
|
Checkpoint Kinase 1 Phospho-Ser296 (CHEK1 pS296) Antibody |
abx324716-100g |
Abbexa |
100 µg |
EUR 250 |
Checkpoint Kinase 1 Phospho-Ser296 (CHEK1 pS296) Antibody |
abx324716-50g |
Abbexa |
50 µg |
EUR 187.5 |
Serine/threonine-protein kinase Chk1 (CHEK1) Antibody |
abx402060-200g |
Abbexa |
200 µg |
EUR 287.5 |
Serine/Threonine Protein Kinase CHK1 (CHEK1) Rabbit anti-Human Polyclonal (Ser280) Antibody |
GWB-35AAD3 |
GenWay Biotech |
0.05 mg |
Ask for price |
|
CHEK1 antibody |
10R-1343 |
Fitzgerald |
100 ug |
EUR 614.4 |
Description: Mouse monoclonal CHEK1 antibody |
CHEK1 Antibody |
46488 |
SAB |
100ul |
EUR 319 |
CHEK1 Antibody |
46488-100ul |
SAB |
100ul |
EUR 302.4 |
CHEK1 Antibody |
1-CSB-PA927149 |
Cusabio |
-
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-
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|
|
|
Description: A polyclonal antibody against CHEK1. Recognizes CHEK1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;ELISA:1:1000-1:2000, WB:1:200-1:1000 |
CHEK1 Antibody |
E046488 |
EnoGene |
100μg/100μl |
EUR 255 |
Description: Available in various conjugation types. |
CHEK1 Antibody |
E92865 |
EnoGene |
100μg |
EUR 255 |
Description: Available in various conjugation types. |
CHEK1 antibody |
70R-13984 |
Fitzgerald |
100 ug |
EUR 386.4 |
Description: Affinity purified Rabbit polyclonal CHEK1 antibody |
CHEK1 antibody |
70R-5597 |
Fitzgerald |
50 ug |
EUR 467 |
|
Description: Rabbit polyclonal CHEK1 antibody |
CHEK1 antibody |
70R-5598 |
Fitzgerald |
50 ug |
EUR 467 |
|
Description: Rabbit polyclonal CHEK1 antibody |
CHEK1 Antibody |
1-CSB-PA005338GA01HU |
Cusabio |
-
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-
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|
|
|
Description: A polyclonal antibody against CHEK1. Recognizes CHEK1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB |
CHEK1 Antibody |
1-CSB-PA005338LA01HU |
Cusabio |
-
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-
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|
|
|
Description: A polyclonal antibody against CHEK1. Recognizes CHEK1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA |
CHEK1 Antibody |
1-CSB-PA006773 |
Cusabio |
-
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-
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|
|
|
Description: A polyclonal antibody against CHEK1. Recognizes CHEK1 from Human. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/10000 |
CHEK1 Antibody |
1-CSB-PA006774 |
Cusabio |
-
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-
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|
|
|
Description: A polyclonal antibody against CHEK1. Recognizes CHEK1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IF, ELISA;WB:1/500-1/2000.IF:1/200-1/1000.ELISA:1/10000 |
CHEK1 Antibody |
1-CSB-PA006775 |
Cusabio |
-
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-
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|
|
|
Description: A polyclonal antibody against CHEK1. Recognizes CHEK1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, IF, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.IF:1/200-1/1000.ELISA:1/10000 |
CHEK1 Antibody |
1-CSB-PA001623 |
Cusabio |
-
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-
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|
|
|
Description: A polyclonal antibody against CHEK1. Recognizes CHEK1 from Human. This antibody is Unconjugated. Tested in the following application: WB, IHC, IF, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.IF:1/200-1/1000.ELISA:1/5000 |
CHEK1 Antibody |
1-CSB-PA001626 |
Cusabio |
-
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-
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|
|
|
Description: A polyclonal antibody against CHEK1. Recognizes CHEK1 from Human. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/5000 |
CHEK1 Antibody |
1-CSB-PA001629 |
Cusabio |
-
Ask for price
-
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|
|
|
Description: A polyclonal antibody against CHEK1. Recognizes CHEK1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/40000 |
CHEK1 Antibody |
K52027M02A04C-100ug |
Absea Biotechnology |
100 ug |
EUR 360 |
|
Description: Monoclonal Antibody |
CHEK1 Antibody |
K52027M02A04C-1mg |
Absea Biotechnology |
1 mg |
EUR 1800 |
|
Description: Monoclonal Antibody |
CHEK1 Antibody |
K52027M02A04C-50ug |
Absea Biotechnology |
50 ug |
EUR 252 |
|
Description: Monoclonal Antibody |
CHEK1 Antibody |
K52027M13E04C-100ug |
Absea Biotechnology |
100 ug |
EUR 320 |
|
Description: Monoclonal Antibody |
CHEK1 Antibody |
K52027M13E04C-1mg |
Absea Biotechnology |
1 mg |
EUR 1600 |
|
Description: Monoclonal Antibody |
CHEK1 Antibody |
K52027M13E04C-50ug |
Absea Biotechnology |
50 ug |
EUR 224 |
|
Description: Monoclonal Antibody |
CHEK1 Antibody |
K52027M20D11C-100ug |
Absea Biotechnology |
100 ug |
EUR 400 |
|
Description: Monoclonal Antibody |
CHEK1 Antibody |
K52027M20D11C-1mg |
Absea Biotechnology |
1 mg |
EUR 2000 |
|
Description: Monoclonal Antibody |
CHEK1 Antibody |
K52027M20D11C-50ug |
Absea Biotechnology |
50 ug |
EUR 280 |
|
Description: Monoclonal Antibody |
CHEK1 Antibody |
MBS7136508-005mL |
MyBiosource |
0.05mL |
EUR 220 |
CHEK1 Antibody |
MBS7136508-01mL |
MyBiosource |
0.1mL |
EUR 300 |
CHEK1 Antibody |
MBS7136508-5x01mL |
MyBiosource |
5x0.1mL |
EUR 1350 |
CHEK1 Antibody |
MBS7126968-005mL |
MyBiosource |
0.05mL |
EUR 190 |
CHEK1 Antibody |
MBS7126968-01mL |
MyBiosource |
0.1mL |
EUR 270 |
CHEK1 Antibody |
MBS7126968-5x01mL |
MyBiosource |
5x0.1mL |
EUR 1205 |
CHEK1 Antibody |
MBS7047913-005mg |
MyBiosource |
0.05mg |
EUR 190 |
CHEK1 Antibody |
MBS7047913-01mg |
MyBiosource |
0.1mg |
EUR 270 |
CHEK1 Antibody |
MBS7047913-5x01mg |
MyBiosource |
5x0.1mg |
EUR 1205 |
CHEK1 Antibody |
MBS7121878-005mg |
MyBiosource |
0.05mg |
EUR 150 |
CHEK1 Antibody |
MBS7121878-01mg |
MyBiosource |
0.1mg |
EUR 190 |
CHEK1 Antibody |
MBS7121878-5x01mg |
MyBiosource |
5x0.1mg |
EUR 845 |
CHEK1 Antibody |
MBS7116126-005mg |
MyBiosource |
0.05mg |
EUR 150 |
CHEK1 Antibody |
MBS7116126-01mg |
MyBiosource |
0.1mg |
EUR 190 |
CHEK1 Antibody |
MBS7116126-5x01mg |
MyBiosource |
5x0.1mg |
EUR 845 |
CHEK1 Antibody |
MBS7116127-005mg |
MyBiosource |
0.05mg |
EUR 150 |
CHEK1 Antibody |
MBS7116127-01mg |
MyBiosource |
0.1mg |
EUR 190 |
CHEK1 Antibody |
MBS7116127-5x01mg |
MyBiosource |
5x0.1mg |
EUR 845 |
CHEK1 Antibody |
MBS7117925-005mg |
MyBiosource |
0.05mg |
EUR 150 |
CHEK1 Antibody |
MBS7117925-01mg |
MyBiosource |
0.1mg |
EUR 190 |
CHEK1 Antibody |
MBS7117925-5x01mg |
MyBiosource |
5x0.1mg |
EUR 845 |
CHEK1 Antibody |
MBS7117926-005mg |
MyBiosource |
0.05mg |
EUR 150 |
CHEK1 Antibody |
MBS7117926-01mg |
MyBiosource |
0.1mg |
EUR 190 |
CHEK1 Antibody |
MBS7117926-5x01mg |
MyBiosource |
5x0.1mg |
EUR 845 |
CHEK1 Antibody |
MBS7117927-005mg |
MyBiosource |
0.05mg |
EUR 150 |
CHEK1 Antibody |
MBS7117927-01mg |
MyBiosource |
0.1mg |
EUR 190 |
CHEK1 Antibody |
MBS7117927-5x01mg |
MyBiosource |
5x0.1mg |
EUR 845 |
CHEK1 Antibody |
MBS8502197-01mg |
MyBiosource |
0.1mg |
EUR 325 |
CHEK1 Antibody |
MBS8502197-01mLAF405L |
MyBiosource |
0.1mL(AF405L) |
EUR 565 |
CHEK1 Antibody |
MBS8502197-01mLAF405S |
MyBiosource |
0.1mL(AF405S) |
EUR 565 |
CHEK1 Antibody |
MBS8502197-01mLAF610 |
MyBiosource |
0.1mL(AF610) |
EUR 565 |
CHEK1 Antibody |
MBS8502197-01mLAF635 |
MyBiosource |
0.1mL(AF635) |
EUR 565 |
CHEK1 Antibody |
MBS9429635-01mL |
MyBiosource |
0.1mL |
EUR 305 |
CHEK1 Antibody |
MBS9429635-5x01mL |
MyBiosource |
5x0.1mL |
EUR 1230 |
Chk1, (Ser280) (CHEK1, Cell Cycle Checkpoint Kinase) |
MBS622709-004mg |
MyBiosource |
0.04mg |
EUR 430 |
Chk1, (Ser280) (CHEK1, Cell Cycle Checkpoint Kinase) |
MBS622709-5x004mg |
MyBiosource |
5x0.04mg |
EUR 1790 |
CHEK1 (pS345) Antibody |
20-abx123327 |
Abbexa |
-
Ask for price
-
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-
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-
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|
- 100 ul
- 200 ul
- 20 ul
- 50 ul
|
|
CHEK1 (pS280) Antibody |
20-abx000106 |
Abbexa |
-
Ask for price
-
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-
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-
Ask for price
|
- 100 ul
- 200 ul
- 20 ul
- 50 ul
|
|
CHEK1 (pS317) Antibody |
20-abx000107 |
Abbexa |
-
Ask for price
-
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-
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-
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|
- 100 ul
- 200 ul
- 20 ul
- 50 ul
|
|
CHEK1 (pS280) Antibody |
20-abx000231 |
Abbexa |
-
Ask for price
-
Ask for price
-
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|
|
|
CHEK1 (pS296) Antibody |
abx332897-100ul |
Abbexa |
100 ul |
EUR 560.4 |
|
CHEK1 (pS286) Antibody |
abx332913-100ul |
Abbexa |
100 ul |
EUR 560.4 |
|
CHEK1 (pS301) Antibody |
abx333367-100ul |
Abbexa |
100 ul |
EUR 560.4 |
|
CHEK1 (pS345) Antibody |
abx123327-100l |
Abbexa |
100 µl |
EUR 200 |
CHEK1 (pS345) Antibody |
abx123327-1ml |
Abbexa |
1 ml |
EUR 475 |
CHEK1 (pS345) Antibody |
abx123327-200l |
Abbexa |
200 µl |
EUR 325 |
CHEK1 (pS296) Antibody |
abx332897-100g |
Abbexa |
100 µg |
Ask for price |
CHEK1 (pS296) Antibody |
abx332897-20g |
Abbexa |
20 µg |
EUR 412.5 |
CHEK1 (pS296) Antibody |
abx332897-50g |
Abbexa |
50 µg |
Ask for price |
CHEK1 (pS286) Antibody |
abx332913-100g |
Abbexa |
100 µg |
Ask for price |
CHEK1 (pS286) Antibody |
abx332913-20g |
Abbexa |
20 µg |
EUR 412.5 |
CHEK1 (pS286) Antibody |
abx332913-50g |
Abbexa |
50 µg |
Ask for price |
CHEK1 (pS301) Antibody |
abx333367-100g |
Abbexa |
100 µg |
Ask for price |
CHEK1 (pS301) Antibody |
abx333367-20g |
Abbexa |
20 µg |
EUR 412.5 |
CHEK1 (pS301) Antibody |
abx333367-50g |
Abbexa |
50 µg |
Ask for price |
CHEK1 (pS280) Antibody |
abx000106-100l |
Abbexa |
100 µl |
EUR 475 |
CHEK1 (pS280) Antibody |
abx000106-20l |
Abbexa |
20 µl |
EUR 200 |
CHEK1 (pS280) Antibody |
abx000106-50l |
Abbexa |
50 µl |
EUR 325 |
CHEK1 (pS317) Antibody |
abx000107-100l |
Abbexa |
100 µl |
EUR 475 |
CHEK1 (pS317) Antibody |
abx000107-20l |
Abbexa |
20 µl |
EUR 200 |
CHEK1 (pS317) Antibody |
abx000107-50l |
Abbexa |
50 µl |
EUR 325 |
CHEK1 Polyclonal Antibody |
E97653 |
EnoGene |
100ul |
EUR 225 |
Description: Available in various conjugation types. |
CHEK1 Conjugated Antibody |
C46488 |
SAB |
100ul |
EUR 476.4 |
CHEK1 Polyclonal Antibody |
E-AB-62039-120uL |
Elabscience Biotech |
120uL |
EUR 320 |
|
Description: Unconjugated |
CHEK1 Polyclonal Antibody |
E-AB-62039-200uL |
Elabscience Biotech |
200uL |
EUR 530 |
|
Description: Unconjugated |
CHEK1 Polyclonal Antibody |
E-AB-62039-60uL |
Elabscience Biotech |
60uL |
EUR 200 |
|
Description: Unconjugated |
CHEK1 Polyclonal Antibody |
E-AB-62039-each |
Elabscience Biotech |
each |
Ask for price |
|
Description: Unconjugated |
CHEK1 Polyclonal Antibody |
MBS9128725-002mL |
MyBiosource |
0.02mL |
EUR 200 |
CHEK1 Polyclonal Antibody |
MBS9128725-005mL |
MyBiosource |
0.05mL |
EUR 255 |
CHEK1 Polyclonal Antibody |
MBS9128725-01mL |
MyBiosource |
0.1mL |
EUR 345 |
CHEK1 Polyclonal Antibody |
MBS9128725-02mL |
MyBiosource |
0.2mL |
EUR 545 |
CHEK1 Polyclonal Antibody |
MBS9128725-5x02mL |
MyBiosource |
5x0.2mL |
EUR 2265 |
CHEK1 Polyclonal Antibody |
MBS8573498-01mL |
MyBiosource |
0.1mL |
EUR 305 |
CHEK1 Polyclonal Antibody |
MBS8573498-01mLAF405L |
MyBiosource |
0.1mL(AF405L) |
EUR 565 |
CHEK1 Polyclonal Antibody |
MBS8573498-01mLAF405S |
MyBiosource |
0.1mL(AF405S) |
EUR 565 |
CHEK1 Polyclonal Antibody |
MBS8573498-01mLAF610 |
MyBiosource |
0.1mL(AF610) |
EUR 565 |
CHEK1 Polyclonal Antibody |
MBS8573498-01mLAF635 |
MyBiosource |
0.1mL(AF635) |
EUR 565 |
CHEK1 Polyclonal Antibody |
MBS2539982-006mL |
MyBiosource |
0.06mL |
EUR 190 |
CHEK1 Polyclonal Antibody |
MBS2539982-012mL |
MyBiosource |
0.12mL |
EUR 265 |
CHEK1 Polyclonal Antibody |
MBS2539982-02mL |
MyBiosource |
0.2mL |
EUR 415 |
CHEK1 Polyclonal Antibody |
MBS2539982-5x02mL |
MyBiosource |
5x0.2mL |
EUR 1835 |
CHEK1 Polyclonal Antibody |
MBS2542709-002mL |
MyBiosource |
0.02mL |
EUR 140 |
CHEK1 Polyclonal Antibody |
MBS2542709-006mL |
MyBiosource |
0.06mL |
EUR 180 |
CHEK1 Polyclonal Antibody |
MBS2542709-012mL |
MyBiosource |
0.12mL |
EUR 260 |
CHEK1 Polyclonal Antibody |
MBS2542709-02mL |
MyBiosource |
0.2mL |
EUR 405 |
CHEK1 Polyclonal Antibody |
MBS2542709-5x02mL |
MyBiosource |
5x0.2mL |
EUR 1725 |
CHEK1 Conjugated Antibody |
MBS9454425-01mLAF350 |
MyBiosource |
0.1mL(AF350) |
EUR 480 |
CHEK1 Conjugated Antibody |
MBS9454425-01mLAF405 |
MyBiosource |
0.1mL(AF405) |
EUR 480 |
CHEK1 Conjugated Antibody |
MBS9454425-01mLAF488 |
MyBiosource |
0.1mL(AF488) |
EUR 480 |
CHEK1 Conjugated Antibody |
MBS9454425-01mLAF555 |
MyBiosource |
0.1mL(AF555) |
EUR 480 |
CHEK1 Conjugated Antibody |
MBS9454425-01mLBiotin |
MyBiosource |
0.1mL(Biotin) |
EUR 480 |
CHEK1 Polyclonal Antibody |
RD84616A-120uL |
Reddot Biotech |
120μL |
EUR 360 |
|
Description: The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. |
CHEK1 Polyclonal Antibody |
RD84616A-200uL |
Reddot Biotech |
200μL |
EUR 630 |
|
Description: The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. |
CHEK1 Polyclonal Antibody |
RD84616A-60uL |
Reddot Biotech |
60μL |
EUR 204 |
|
Description: The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. |
Serine/Threonine-Protein Kinase CHK1 Phospho-Ser280 (CHEK1 pS280) Antibody |
20-abx012528 |
Abbexa |
-
Ask for price
-
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-
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-
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|
- 100 ug
- 10 ug
- 200 ug
- 300 µg
|
|
Serine/Threonine-Protein Kinase CHK1 Phospho-Ser280 (CHEK1 pS280) Antibody |
abx012528-100g |
Abbexa |
100 µg |
EUR 237.5 |
Serine/Threonine-Protein Kinase CHK1 Phospho-Ser280 (CHEK1 pS280) Antibody |
abx012528-10g |
Abbexa |
10 µg |
EUR 43.75 |
Serine/Threonine-Protein Kinase CHK1 Phospho-Ser280 (CHEK1 pS280) Antibody |
abx012528-200g |
Abbexa |
200 µg |
EUR 312.5 |
Chk1, phosphorylated (Ser345) (CHEK1, Cell Cycle Checkpoint Kinase) |
MBS620911-01mL |
MyBiosource |
0.1mL |
EUR 695 |
Chk1, phosphorylated (Ser345) (CHEK1, Cell Cycle Checkpoint Kinase) |
MBS620911-5x01mL |
MyBiosource |
5x0.1mL |
EUR 2985 |
Chk1, phosphorylated (Ser317) (CHEK1, Cell Cycle Checkpoint Kinase) |
MBS622828-01mL |
MyBiosource |
0.1mL |
EUR 695 |
Chk1, phosphorylated (Ser317) (CHEK1, Cell Cycle Checkpoint Kinase) |
MBS622828-5x01mL |
MyBiosource |
5x0.1mL |
EUR 2985 |
CHEK1 (Ab-280) Antibody |
CSB-PA584688- |
Cusabio |
each |
EUR 402 |
|
Description: A polyclonal antibody against CHEK1 (Ab-280). Recognizes CHEK1 (Ab-280) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
CHEK1 (Ab-280) Antibody |
CSB-PA584688-100ul |
Cusabio |
100ul |
EUR 379.2 |
|
Description: A polyclonal antibody against CHEK1 (Ab-280). Recognizes CHEK1 (Ab-280) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
CHEK1 (Ab-317) Antibody |
CSB-PA245196- |
Cusabio |
each |
EUR 402 |
|
Description: A polyclonal antibody against CHEK1 (Ab-317). Recognizes CHEK1 (Ab-317) from Human, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:1000, IHC:1:50-1:200 |
CHEK1 (Ab-317) Antibody |
CSB-PA245196-100ul |
Cusabio |
100ul |
EUR 379.2 |
|
Description: A polyclonal antibody against CHEK1 (Ab-317). Recognizes CHEK1 (Ab-317) from Human, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:1000, IHC:1:50-1:200 |
CHEK1 (Ab-280) Antibody |
MBS7130604-01mL |
MyBiosource |
0.1mL |
EUR 270 |
CHEK1 (Ab-280) Antibody |
MBS7130604-5x01mL |
MyBiosource |
5x0.1mL |
EUR 1200 |
CHEK1 (Ab-317) Antibody |
MBS7131926-01mL |
MyBiosource |
0.1mL |
EUR 270 |
CHEK1 (Ab-317) Antibody |
MBS7131926-5x01mL |
MyBiosource |
5x0.1mL |
EUR 1200 |
Anti-Chk1/CHEK1 Antibody |
A01060 |
BosterBio |
100ug/vial |
EUR 352.8 |
Anti-Chk1/CHEK1 Antibody |
PA1445 |
BosterBio |
100ug/vial |
EUR 352.8 |
CHEK1 Antibody (Center S296) |
MBS9204521-008mL |
MyBiosource |
0.08mL |
EUR 210 |
CHEK1 Antibody (Center S296) |
MBS9204521-04mL |
MyBiosource |
0.4mL |
EUR 430 |
CHEK1 Antibody (Center S296) |
MBS9204521-5x04mL |
MyBiosource |
5x0.4mL |
EUR 1910 |
Mouse Chek1 Antibody (Center) |
MBS9232417-008mL |
MyBiosource |
0.08mL |
EUR 210 |
Mouse Chek1 Antibody (Center) |
MBS9232417-04mL |
MyBiosource |
0.4mL |
EUR 430 |
Mouse Chek1 Antibody (Center) |
MBS9232417-5x04mL |
MyBiosource |
5x0.4mL |
EUR 1910 |
CHEK1 Antibody - middle region |
MBS3200221-01mL |
MyBiosource |
0.1mL |
EUR 400 |
CHEK1 Antibody - middle region |
MBS3200221-5x01mL |
MyBiosource |
5x0.1mL |
EUR 1760 |
CHEK1 antibody - middle region |
MBS3201035-01mL |
MyBiosource |
0.1mL |
EUR 455 |
CHEK1 antibody - middle region |
MBS3201035-5x01mL |
MyBiosource |
5x0.1mL |
EUR 1995 |
CHEK1 antibody - middle region |
MBS3201036-01mL |
MyBiosource |
0.1mL |
EUR 455 |
CHEK1 antibody - middle region |
MBS3201036-5x01mL |
MyBiosource |
5x0.1mL |
EUR 1995 |
CHEK1 Antibody - middle region |
MBS3218640-01mL |
MyBiosource |
0.1mL |
EUR 455 |
CHEK1 Antibody - middle region |
MBS3218640-5x01mL |
MyBiosource |
5x0.1mL |
EUR 1995 |
CHEK1 Antibody, HRP conjugated |
1-CSB-PA005338LB01HU |
Cusabio |
-
Ask for price
-
Ask for price
|
|
|
Description: A polyclonal antibody against CHEK1. Recognizes CHEK1 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
The nucleotidyl transferase exercise of Taq polymerase was additionally noticed and resulted within the addition of dUMPs labelled with electroneutral or positively charged fluorophores to the three’ ends of DNA. The introduction of mutually compensating expenses into fluorophores or different practical teams conjugated to dNTPs might be thought of a foundation for the creation of PCR-compatible modified nucleoside triphosphates.
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