Category: Panel

Purification and assembly of thermostable Cy5 labeled γ-PNAs into a 3D DNA nanocage.

PNA is hybrid molecule ideally suited to bridging the useful panorama of polypeptides with the structural range that may be engineered with DNA nanostructures. Nonetheless, PNA could be more difficult to work with in aqueous solvents attributable to its hydrophobic nature. An answer part methodology utilizing pressure promoted, copper free click on chemistry was developed

Read More
Cy3 and Cy5 dyes attached to oligonucleotide terminus stabilize DNA duplexes: predictive thermodynamic model.

Cyanine dyes are vital chemical modifications of oligonucleotides exhibiting intensive and secure fluorescence at seen mild wavelengths. When Cy3 or Cy5 dye is connected to five’ finish of a DNA duplex, the dye stacks on the terminal base pair and stabilizes the duplex. Utilizing optical melting experiments, we’ve decided thermodynamic parameters that may predict the

Read More
Tubular J-aggregates of a new thiacarbocyanine Cy5 dye for the far-red spectral region – a spectroscopic and cryo-transmission electron microscopy study.

The aggregation conduct of a phenol-substituted thiacarbocyanine Cy5 dye (5-chloro-2-[5-[5-chloro-3-(4-sulfobutyl)-3H-benzothiazol-2-ylidene]-3-phenyl-penta-1,3-dienyl]-3-(4-sulfobutyl)-benzothiazol-3-ium hydroxide, interior salt, triethylammonium salt) in aqueous answer is investigated utilizing steady-state absorption, linear dichroism, and fluorescence spectroscopies, in addition to cryogenic transmission electron microscopy (cryo-TEM). By growing the focus, the dye self-assembles in pure water into dimers and H-aggregates, the latter being uniform particles

Read More
Substituent Effects on the Solubility and Electronic Properties of the Cyanine Dye Cy5: Density Functional and Time-Dependent Density Functional Theory Calculations

The aggregation capability and exciton dynamics of dyes are largely affected by properties of the dye monomers. To facilitate aggregation and enhance excitonic perform, dyes may be engineered with substituents to exhibit optimum key properties, similar to hydrophobicity, static dipole second variations, and transition dipole moments. To find out how electron donating (D) and electron

Read More