Category: Enzymes

Cy3 and Cy5 dyes attached to oligonucleotide terminus stabilize DNA duplexes: predictive thermodynamic model.

Cyanine dyes are vital chemical modifications of oligonucleotides exhibiting intensive and secure fluorescence at seen mild wavelengths. When Cy3 or Cy5 dye is connected to five’ finish of a DNA duplex, the dye stacks on the terminal base pair and stabilizes the duplex. Utilizing optical melting experiments, we’ve decided thermodynamic parameters that may predict the

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Substituent Effects on the Solubility and Electronic Properties of the Cyanine Dye Cy5: Density Functional and Time-Dependent Density Functional Theory Calculations

The aggregation capability and exciton dynamics of dyes are largely affected by properties of the dye monomers. To facilitate aggregation and enhance excitonic perform, dyes may be engineered with substituents to exhibit optimum key properties, similar to hydrophobicity, static dipole second variations, and transition dipole moments. To find out how electron donating (D) and electron

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